BMC Genomics. ;11:176. doi: 10.1186/1471-2164-11-176. The embryonic genes Dkk3, Hoxd8, Hoxd9 and Tbx1 identify muscle types in at-independent and fiber-type unrelated way. de Wilde J 1 , Hulshof MF, Boeksc.en MV, de Groot P, Smit E, Mariman EC. Author information: 1 Top Ins.ute Food and .Exp Neurol. 1981 Sep;73 3 :831-6. Enzymic markers to identify muscle-nerve formation during embryogenesis: modified myosin ATPase and silver-cholinesterase histochemical reactions. Butler J, Cosmos E. PMID: 6455302; [Indexed for MEDLINE]. Publication Types: Research Support, Non-U.S. Gov 't. MeSH terms..A rapid and reliable technique is described which permits an investigation of muscle fiber type differentiation using cryostat sections of frozen chick embryos from the primary somite stage 2 days in ovo onward. Histochemical reactions to identify muscle phenotypes myosin ATPase and the presence of nerves .Strategies to identify muscle fatigue from SEMG during cycling. Abstract: Detection, quantification andysis of muscle fatigue are crucial in occupational/rehabilitation and sporting settings. Sports organizations, such as the Australian Ins.ute of Sports AIS , currently monitor fatigue by a battery of tests including invasive .