Identifying Novel Microbial Catalyst By Enrichment Culture And

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- A Novel Reductive Dehalogenase, Identified in a Contaminated Groundwater Enrichment Culture and in Desulfitobacterium dichloroeliminans Strain DCA1 The key catalysts in dehalorespiration are reductive dehalogenases RDs , mem.ne-.ociated enzymes with low levels of nucleotide iden.y but .An enrichment culture in LB medium containing a synthetic substrate d-Phe 4 led to the isolation of a bacterial strain Bacillus cereus DF4-B. The extracellular . The sequence-based methods therefore uncover new variants of already-known enzyme families, but usually fail to identify truly novel genes without related .The use of prokaryote pure cultures, many of which have been obtained by enrichment culture, has been instrumental in the identification of the many novel and microbial phylogenetic diversity. per million when coupled with oxygen-scrubbers for the gas mixtures and catalyst cartridges inside the anaerobic chamber..The traditional method to identify new enzymes is screening of environmental samples e.g. soil samples or strain collections by enrichment culture Asano, 2002; Ogawa and Shimizu, 2002 . Once a suitable biocatalyst is identified, strain improvement as well as cloning and expression of the encoding gene enables .

In this study, a novel bacterial mixed culture AC, capable of degrading aniline, was developed by us. The mixed culture presented better aniline degradation abilities .Application of met.omic techniques in mining enzymes from microbial communities for biofuel synthesis.

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