Identifying Genes Functional Cloning

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This method byp.ed the protein and enabled direct isolation of genes on the basis of their chromosomal location and certain characteristics that identified a segment of DNA as "gene-like." Identification of the mutant gene as well as knowledge of the genetic disorder could then be inferred from the DNA sequence..

Functional Gene Cloning. Functional cloning has been used to identify genes causing human diseases such as phenylketonuria and sickle cell ane .Functional cloning involves using a known gene sequence as a probe to look for new gene sequences that may have similar functions, based on the similarity of sequence. Using this method ofysis, researchers can search through entire genomes to find genes of interest without having prior knowledge of their location..Identification of disease-related genes identifying genes B. Positional cloning of disease genes Functional data are not.CHAPTER 5 Mapping and Identifying Genes and even whole genome sequencing are now feasible strategies for identifying disease genes by Functional cloning .

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