Unknown bacteria are first collected by swabbing surfaces around and near the lab, then streaked on sterile LB agar plates and grown overnight in an incubator. A different "unknown number" is given to each place bacteria are collected. Students then use these plates to make their own "unknown plate" by streaking for single colonies..Unknowns - 1 IDENTIFICATION OF UNKNOWNS In this exercise, you will draw upon many of your new skills to identify unknown bacteria. You will be given three bacterial cultures and asked to identify each to the level of genus..Objective: To identify the bacterial unknowns in a mixed culture by morphological and biochemical methods. Principle: The identification of bacteria is a careful and systematic process that uses many different techniques to narrow down the types of bacteria that are present in an unknown bacterial culture..Whether it is to determine the cause of a disease, to test if a certain food is safe to eat or simply to know what is present in a certain ecosystem, we have developed many techniques to identify bacteria. Bacteria may seem like very simple organisms and you might think that most of them share many characteristics. In fact, every species is .
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